Composite

Part:BBa_K848002

Designed by: Antoine Ribeiro   Group: iGEM12_Bordeaux   (2012-09-26)


luxI-lacI-mCherry-dbl terminator

We added a double terminator to the intermediate part BBa_S03816 in order to make it a transcriptional unit ready to use. This part Produce LuxI and LacI signal molecules with a mCherry reporter. LuxI is a quorum sensing protein that converts SAM into acyl-homoserine lactone (AHL) thus acting as pLuxR promoter activator when bound to LuxR protein (BBa_K329007). LacI binds to the pLac regulator (BBa_R0010) and PLac01 hybrid regulator (BBa_R0011) inhibits transcription. IPTG binds to LacI and inhibits its operation, therefore promoting transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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