Part:BBa_K845002

Strong RBS GFPmut2

RBS shine d'algarno

GFP mut2 (S65A V68L S72A): this variant used becomes fluorescent within less than five minutes of transcription initiation, and is highly stable and nonetoxic in E. Coli.

Usage and Biology

Used as a reporter (fluorescence). Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 670