Regulatory

Part:BBa_K844010

Designed by: Kathleen Miller   Group: iGEM12_Utah_State   (2012-10-03)

Enhanced tRNA Promoter for ''E. coli''

Usage and Biology

This part is a mutated version of the E. coli leuV tRNA promoter. This part is based on the ZH14 sequence from Bauer et al. (1993). This promoter has approximately normal growth-rate dependent regulation but 12.0x normal promoter strength in rich medium.

It is important to use a tRNA promoter to express tRNA parts, as other promoters will recruit RNA polymerase II, which only synthesizes mRNA. The tRNA promoter will recruit RNA polymerase III, which is the correct polymerase for synthesizing tRNAs.


Related Parts

BBa_K8440011 - This part is a tRNA specific terminator for E. coli


References

Bauer B.F., Elford R.M., and Holmes W.M. 1993. Mutagenesis and functional analysis of the Escherichia coli tRNA (1Leu) promoter. Mol microbio. 7:265–273.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None