Tag

Part:BBa_K844000:Experience

Designed by: Kathleen Miller   Group: iGEM12_Utah_State   (2012-10-02)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K844000

User Reviews

UNIQ68481597c6f41726-partinfo-00000000-QINU

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2012 Utah_State iGEM Team

This part functions as expected: it is capable of binding to Nickel resin columnns with high affinity, and it capable of being recognized by histidine-tag specific antibodies and dyes. See the main part page for gel photo and details.

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UNIQ68481597c6f41726-partinfo-00000002-QINU UNIQ68481597c6f41726-partinfo-00000003-QINU

Utah State iGEM team 2013 had good results using this 10x His-Tag for the purification of antimicrobial spider silk from E. coli BBa_K1162306.