Composite

Part:BBa_K843008

Designed by: Alişan Kayabölen   Group: iGEM12_METU   (2012-10-02)


T4 Lysis Device under control of IPTG

By using this composite part, it is possible to keep living of bacteria under control of IPTG. This part contains a Kill Switch lysis machine designed by iGEM08 UC Berkeley team, with a cI regulated promoter. Also, there is a cI coding region with a LacI regulated promoter.

By this way, in a medium containing IPTG, LacI will be inhibited by IPTG. Therefore, LacI regulated promoter will be active and cI protein will be produced. As a result, cI protein will inhibit cI regulated promoter activity. So, kill switch part (T4 Lysis Device) will not work. In contrast, in the absence of IPTG, LacI inhibits its promoter, and so cI will not be produced. Therefore, cI regulated promoter will be active and T4 Lysis Device take bacteria to the lysis.

As a result, this composite part has a big potential to be used as a safety device.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1427
    Illegal NheI site found at 1450
    Illegal NheI site found at 1475
    Illegal NheI site found at 1498
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1032
    Illegal AgeI site found at 1102
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None