Device

Part:BBa_K843007

Designed by: Berke Gürkan   Group: iGEM12_METU   (2012-09-26)


Cell Limiter using quorum sensing

The device inhibits cell division after it reaches a critical level. For this we used MinC as the cell division inhibitor. The critical level can be changed by changing the promoter before the LuxI region.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2685
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 2685
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 698
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2685
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2685
    Illegal AgeI site found at 2244
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 847
    Illegal BsaI.rc site found at 1950


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