Part:BBa_K842012

motB

motB is a structural protein for the stationary element of the flagella motor. The protein works in conjunction with motA to form this structure, which then in turn controls the flagella’s rotation. It is also believed that motB is responsible for forming the structure that connects the flagella motor to the cell wall.

Used for reconstitution of flagella apparatus in E. coli B strains.

Cloning and Uses This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. The protein works in conjunction with motA to form the stationary base of the flagella motor, which then in turn controls the flagella’s rotation. This part can be used in the complete recreation of an E. coli’s genetic flageller pathway.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

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