Translational_Unit
nitR

Part:BBa_K836007

Designed by: Ricardo Alvarado & Veronica Delgado   Group: iGEM12_Costa_Rica-TEC-UNA   (2012-09-20)


Nitrilase regulator from R. rhodochrous (codon usage optimized for R. opacus)

Protein in charge to regulate the expression of the genes put under control of nitA promoter. A Gram positive RBS (SpoVG https://parts.igem.org/wiki/index.php?title=Part:BBa_K143021) was inserted at the beginning.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 640
    Illegal XhoI site found at 796
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 274
    Illegal NgoMIV site found at 532
    Illegal NgoMIV site found at 833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 45
    Illegal BsaI site found at 492
    Illegal BsaI site found at 969


[edit]
Categories
Parameters
None