Translational_Unit
gpS

Part:BBa_K836004

Designed by: Ricardo Alvarado   Group: iGEM12_Costa_Rica-TEC-UNA   (2012-09-20)

Lysis inhibitor from Enterobacteria phage lambda (codon usage optimized for R. opacus)

This protein is the one in charge to avoid the formation of pores in the inner membrane of the bacterial cell. It has been optimized for expression in R. opacus. A weak RBS for Gram positive bacteria was used (https://parts.igem.org/Part:BBa_K090506:Design) in order to obtain low concentration of the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 194
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/lysis
//cds/membrane/lysis
//function/celldeath
Parameters
None