Coding
DGAT

Part:BBa_K836002

Designed by: Ricardo Alvarado   Group: iGEM12_Costa_Rica-TEC-UNA   (2012-09-18)


O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) as used

This biobrick codifies for the protein DGAT (Diacylglycerol O-acyltransferase) which have dual activity, it can act as a wax ester synthase or as a diacylglycerol acyltransferase. In the latter case, its function consists in mediate the final step in TGAs synthesis using a fatty acyl-CoA and a DGA. This enzyme is vital in the storage and synthesis of lipids.

Based on Uniprot information, this enzyme is more likely to act as a wax ester synthase with (C12-C18)-CoA whereas with (C20)-CoA it acts as a DGAT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 40
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 418
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 192


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
Parameters
None