Coding

Part:BBa_K833013

Designed by: Julianne Grose   Group: iGEM12_BYUProvo   (2012-10-02)

Lactate sensor derived from the AraF protein of E. coli

This part is a lactate sensor derived from site directed mutagenesis of the E. coli AraF protein. This part is a periplasmic lactate sensor derived from site directed mutagenesis of the E. coli AraF protein to create the lactate sensor described by Looger et al. (Looger, LL. et al., Computational design of receptor and sensor proteins with novel functions (Nature 2003). TDue to the multiple mutations, two rounds of mutagenesis were necessary to incorporate all mutations, and several colonies had to be sequenced after each round in order to identify colonies which contained all of the mutations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 106


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