Plasmid_Backbone

Part:BBa_K823055

Designed by: Jara Radeck   Group: iGEM12_LMU-Munich   (2013-10-16)


pSB1C3F-Vector pSB1C3 with RFP-cassette in Freiburg Standard

To allow the possibility to save some nucleotides of primer-overhangs, you can use this vector. The two AgeI-restriction sites from the original RFP-cassette have been removed. The cassette is flanked by the modified Freiburg standard (http://2012.igem.org/wiki/images/b/b9/LMU-Munich_2012_Our_Freiburg_standard_FusionPrefix.pdf) that includes an optimal ribosome binding site between XbaI and NgoMIV restriction sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal suffix found at 10
    Illegal EcoRI site found at 2058
    Illegal XbaI site found at 2073
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2058
    Illegal SpeI site found at 11
    Illegal PstI site found at 25
    Illegal NotI site found at 18
    Illegal NotI site found at 2064
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2058
    Illegal XhoI site found at 1042
    Illegal XhoI site found at 1934
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2058
    Illegal suffix found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2058
    Illegal XbaI site found at 2073
    Illegal NgoMIV site found at 2097
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/high
Parameters
chassis
copies100-300
insertNone
mcsBioBrick
originpMB1
resistanceC