Part:BBa_K823055

pSB1C3F-Vector pSB1C3 with RFP-cassette in Freiburg Standard

To allow the possibility to save some nucleotides of primer-overhangs, you can use this vector. The two AgeI-restriction sites from the original RFP-cassette have been removed. The cassette is flanked by the modified Freiburg standard (https://static.igem.org/mediawiki/2012/b/b9/LMU-Munich_2012_Our_Freiburg_standard_FusionPrefix.pdf) that includes an optimal ribosome binding site between XbaI and NgoMIV restriction sites.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Illegal suffix found at 10
  Illegal EcoRI site found at 2058
  Illegal XbaI site found at 2073
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2058
  Illegal SpeI site found at 11
  Illegal PstI site found at 25
  Illegal NotI site found at 18
  Illegal NotI site found at 2064
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2058
  Illegal XhoI site found at 1042
  Illegal XhoI site found at 1934
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 2058
  Illegal suffix found at 11
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 2058
  Illegal XbaI site found at 2073
  Illegal NgoMIV site found at 2097
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.