Regulatory

Part:BBa_K821004

Designed by: Jinlai Zhang, Dongchang Qin   Group: iGEM12_Tianjin   (2012-09-17)


PBad Promoter

We originally used the PBad promoter from the distribution kit. But all of them failed to work. So we extracted this PBad promoter from pKD46 on our own, and tested it in our cells. The result is perfect.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1095
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1077


[edit]
Categories
Parameters
None