Device

Part:BBa_K817019

Designed by: Shan Chi Hsieh   Group: iGEM12_NTU-Taida   (2012-09-18)


parAB operon from pseudomonas putida KT2440 chromosome

When providing this part in cis or in trans, it can active partition the low copy plasmid with parS site thus it can enhances plasmid segregation stability and reduces plasmid loss rate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1593
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1593
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1593
    Illegal XhoI site found at 519
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1593
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1593
    Illegal NgoMIV site found at 98
    Illegal NgoMIV site found at 608
    Illegal NgoMIV site found at 1455
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 822
    Illegal BsaI site found at 1173
    Illegal SapI.rc site found at 1227
    Illegal SapI.rc site found at 1335


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Categories
Parameters
None