Device

Part:BBa_K817017

Designed by: Shan Chi Hsieh   Group: iGEM12_NTU-Taida   (2012-09-18)

pSB2K3 with parS site

pSB2K3 is mini F plasmid that lacks partition system and replicates as low copy in cell. pSB2K3 with parS site can be partitioned by parAB cassette which can enhances its segregation stability.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4404
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4404
    Illegal NheI site found at 3317
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4410
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4404
    Illegal BglII site found at 1618
    Illegal BglII site found at 2665
    Illegal XhoI site found at 3462
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4404
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4404
    Illegal XbaI site found at 4419
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1061
    Illegal NgoMIV site found at 3303
    Illegal AgeI site found at 937
    Illegal AgeI site found at 2596
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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