Device

Part:BBa_K817015

Designed by: Shan Chi Hsieh   Group: iGEM12_NTU-Taida   (2012-09-18)


srnBC toxin-antitoxin cassette

It is type I toxin-antitoxin which belongs to hok/sok homologues, it expresses stable toxin encoded RNA and short lived antitoxin RNA that can neutralize toxin RNA by RNA interaction. It acts as post segregation killing system, which kills bacteria when it loss the DNA(genomic islands, plasmids, mobile gene elements) that contains it. Therefore we use it to reduce plasmid loss rate and make applications without antibiotics selection pressure more feasible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 524
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None