Generator

Part:BBa_K814009

Designed by: Sarah Bloch   Group: iGEM12_Minnesota   (2012-10-03)

ScyC generator

Our research has focused onnovel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyC (NpR1276) is a protein of unknown function which converts the ketoacid generated by ScyA into an indole compound which spontaneously dimerizes to form scytonemin.

This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyC. This part can be used to express ScyC protein in E. coli.

Balskus and Walsh, 2009.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2766776/]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2015
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 134
    Illegal XhoI site found at 2023
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 794
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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