Part:BBa_K814006
negative control for mycosporine-like amino acid biosynthesis
Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate. Dehydroquinate-O-methyltransferase (O-MT) then generates 4-deoxygadusol, which is converted to mycosprine-glycine by ATP-grasp (ATPG). Finally, mycosporine-glycine is converted into shinorine by a non-ribosomal peptide synthase (NRPS).
This composite part contains protein generators for DHQS and ATPG. The part is non-functional and was used by the 2012 Minnesota iGEM team as a negative control when testing biosynthesis of mycosporine-like amino acids by the parts BBa_K814004 and BBa_K814005.
Figure 1. HPLC trace showing the DA negative control lacking production of UV-protectant compounds. In both trace figures, the negative control (DA) is shown below the corresponding trace for UV-protectant compound production.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 250
Illegal EcoRI site found at 2397 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 250
Illegal EcoRI site found at 2397
Illegal NotI site found at 1373
Illegal NotI site found at 2970 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 250
Illegal EcoRI site found at 2397
Illegal BglII site found at 134
Illegal BglII site found at 1587
Illegal XhoI site found at 1381
Illegal XhoI site found at 2978 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 250
Illegal EcoRI site found at 2397 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 250
Illegal EcoRI site found at 2397 - 1000COMPATIBLE WITH RFC[1000]
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