Other

Part:BBa_K813002

Designed by: Morgan Baltz, Katie Lutes, Akshitha Yarrabothula, Michael Wierzbicki   Group: iGEM12_UTK-Knoxville   (2012-10-01)

HAP4 - Yeast Genomic IRES

IRES elements initiate 5'- independent translation. Obtained from Saccharomyces cerevisiae (BY4741) genomic DNA.

Zhou, W., Edelman, G. M., & Mauro, V. P. (2001). Transcript leader regions of two Saccharomyces cerevisiae mRNAs contain internal ribosome entry sites that function in living cells. Proceedings of the National Academy of Sciences of the United States of America, 98(4), 1531–6. doi:10.1073/pnas.98.4.1531

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 183


[edit]
Categories
Parameters
None