Coding

Part:BBa_K812120

Designed by: Iryna Nikolayeva   Group: iGEM12_Evry   (2012-09-20)

IaaH on PSC2+ Ready for use in xenopus tropicalis egg


*** WARNING : THIS PART HAS BEEN MERGED WITH BBa_K812020. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES BBa_K812000.***

Corresponds to : This part contains the coding sequence for Indoleacetamide hydrolyase (IaaH). This part is from BBa_K515100, but it was modified to increase the expression in eukaryotic cells by adding a Kozak sequence in front of the original sequence.

This part have the plasmid backbone BBa_K8120000 and the part J04450 is replaced by IaaH BBa_K812000.

This plasmid is ready for injection in tadpole.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1025
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None