Generator

Part:BBa_K812052

Designed by: Cyrille Pauthenier   Group: iGEM12_Evry   (2012-09-20)


pLac promoter R0010 with GoldenBrick extension

This part is the GoldenBricked version of R0010 pLac promoter. The sequence is the same but the biobrick sequence have been modified to enable GoldenBrick assembly, adding a BsaI site and the possibility of BbsI digestion of the scar once formed.

To learn more about the GoldenBrick assembly our team have invented this year, go to: http://2012.igem.org/Team:Evry/GB

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 17
    Illegal SpeI site found at 237
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 237
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 17
    Illegal SpeI site found at 237
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 17
    Illegal SpeI site found at 237
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 231


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