Coding

Part:BBa_K810000

Designed by: Spencer R Katz   Group: iGEM12_Yale   (2012-09-28)


Lambda phage beta CDS with T5 promoter

This part features the coding sequence for the recombinase of the bacteriophage lambda, called beta. It has been fused with a promoter designed by Bryksin and Matsumura (2010) that has been shown to promote transcription in both Gram-positive and Gram-negative organisms. It is flanked by XbaI and SpeI and features a KpnI restriction enzyme site between the promoter and coding sequence. It was designed to go in plasmid pBAV1K (Bryksin and Matsumura) which replicates in both Gram-positive and Gram-negative bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 636
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 636
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 636
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 636
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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