Coding

Part:BBa_K809724

Designed by: Jianhui Gong   Group: iGEM12_Shenzhen   (2012-09-25)

GAL promoter + kozak + Bax(human) + ADH1 terminator

Bax has the same function with holin here. you can get the information of this in BBa_K809723.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2940
    Illegal PstI site found at 3306
    Illegal PstI site found at 3456
    Illegal PstI site found at 3527
    Illegal PstI site found at 5278
    Illegal PstI site found at 5544
    Illegal PstI site found at 6097
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2940
    Illegal PstI site found at 3306
    Illegal PstI site found at 3456
    Illegal PstI site found at 3527
    Illegal PstI site found at 5278
    Illegal PstI site found at 5544
    Illegal PstI site found at 6097
    Illegal NotI site found at 1994
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1278
    Illegal BglII site found at 3490
    Illegal BamHI site found at 2133
    Illegal BamHI site found at 5127
    Illegal BamHI site found at 8006
    Illegal XhoI site found at 1509
    Illegal XhoI site found at 2292
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2940
    Illegal PstI site found at 3306
    Illegal PstI site found at 3456
    Illegal PstI site found at 3527
    Illegal PstI site found at 5278
    Illegal PstI site found at 5544
    Illegal PstI site found at 6097
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2940
    Illegal PstI site found at 3306
    Illegal PstI site found at 3456
    Illegal PstI site found at 3527
    Illegal PstI site found at 5278
    Illegal PstI site found at 5544
    Illegal PstI site found at 6097
    Illegal NgoMIV site found at 2120
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2387
    Illegal BsaI site found at 4603
    Illegal BsaI site found at 5401
    Illegal BsaI site found at 5424
    Illegal BsaI site found at 5494
    Illegal BsaI site found at 5873
    Illegal BsaI site found at 5911
    Illegal BsaI.rc site found at 6261
    Illegal BsaI.rc site found at 6727


[edit]
Categories
Parameters
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