Coding

Part:BBa_K809723

Designed by: Jianhui Gong   Group: iGEM12_Shenzhen   (2012-09-25)

GAL promoter + kozak + hoiln(lambda phage)+ ADH1 terminator

This can identify the function of holin to test if it can anchor on outer membrane of mt and ER only to trigger apoptosis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1278
    Illegal XhoI site found at 1509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None