Coding

Part:BBa_K809722

Designed by: Jianhui Gong   Group: iGEM12_Shenzhen   (2012-09-25)

DLD3 promoter + kozak + yeast GFP + hsl1 tag +ADH1 terminator

This is made to identify the strength of hsl tag to know that if it can succefully extend the working time of YAO.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 170
    Illegal BglII site found at 185
    Illegal BglII site found at 1548
    Illegal BglII site found at 1563
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1294


[edit]
Categories
Parameters
None