Coding

Part:BBa_K809720

Designed by: Jianhui Gong   Group: iGEM12_Shenzhen   (2012-09-25)

DLD3 promoter + kozak + yeast GFP+ ADH1 terminator

This is made to get the basal expression of holin downstream DLD3 promoter and figure out how long yeast can suivive.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1278
    Illegal XhoI site found at 1509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2615


[edit]
Categories
Parameters
None