Coding

Part:BBa_K809719

Designed by: Jianhui Gong   Group: iGEM12_Shenzhen   (2012-09-25)

DLD3 promoter + holin(lambda phage) + hsl1 tag + ADH1 terminator

This is part of suicide of YAO. Holin can form pores on outer membrane of mt and ER to trigger apoptosis of yeast cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1278
    Illegal BglII site found at 2467
    Illegal BglII site found at 2482
    Illegal XhoI site found at 1509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None