Coding

Part:BBa_K809716

Designed by: Jianhui Gong   Group: iGEM12_Shenzhen   (2012-09-25)

GAL promoter + DNase + ADH1 terminator

It can verify the function of DNase I in cytoplasm.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 652
    Illegal PstI site found at 1050
    Illegal PstI site found at 2072
    Illegal PstI site found at 2579
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 652
    Illegal PstI site found at 1050
    Illegal PstI site found at 2072
    Illegal PstI site found at 2579
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 652
    Illegal BglII site found at 868
    Illegal BglII site found at 1157
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 652
    Illegal PstI site found at 1050
    Illegal PstI site found at 2072
    Illegal PstI site found at 2579
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 652
    Illegal PstI site found at 1050
    Illegal PstI site found at 2072
    Illegal PstI site found at 2579
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1282
    Illegal BsaI site found at 1440
    Illegal BsaI site found at 2989
    Illegal BsaI.rc site found at 1104


[edit]
Categories
Parameters
None