Coding

Part:BBa_K809715

Designed by: Jianhui Gong   Group: iGEM12_Shenzhen   (2012-09-25)

T7 promoter + yeast mt RBS(AI1)+ Bovine Pancreatic DNase I (mt)

This is suicide part of project YAO, it can dysfunction the yeast mt.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 186
    Illegal PstI site found at 584
    Illegal PstI site found at 1606
    Illegal PstI site found at 2113
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 186
    Illegal PstI site found at 584
    Illegal PstI site found at 1606
    Illegal PstI site found at 2113
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 186
    Illegal BglII site found at 402
    Illegal BglII site found at 691
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 186
    Illegal PstI site found at 584
    Illegal PstI site found at 1606
    Illegal PstI site found at 2113
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 186
    Illegal PstI site found at 584
    Illegal PstI site found at 1606
    Illegal PstI site found at 2113
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 816
    Illegal BsaI site found at 974
    Illegal BsaI site found at 2523
    Illegal BsaI.rc site found at 638


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Parameters
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