Coding

Part:BBa_K809713

Designed by: Jianhui Gong   Group: iGEM12_Shenzhen   (2012-09-25)

T7 promoter + kozak + GFP + ADH1 terminator

Coupling with BBa_K809712 to identify the function of T7 RNAP/T7 promoter signal imitation system in cytoplasm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 699


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Categories
Parameters
None