Coding

Part:BBa_K809603

Designed by: Jianhui Gong   Group: iGEM12_Shenzhen   (2012-09-24)

Bovine Pancreatic DNase I (in yeast mt codon table)

Bovine Pancreatic DNase I, we change the codon in yeast mt codon table, allowing it to be produced in yeast mitochondria. This is used to fragmentate the mt DNA to make mt dysfunction(mimic the respiration dysfunction) to trigger RTG pathway(retrograde signaling from mt to nucleus) to transport the signal to host cell.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 95
    Illegal PstI site found at 493
    Illegal PstI site found at 1515
    Illegal PstI site found at 2022
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 95
    Illegal PstI site found at 493
    Illegal PstI site found at 1515
    Illegal PstI site found at 2022
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 95
    Illegal BglII site found at 311
    Illegal BglII site found at 600
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 95
    Illegal PstI site found at 493
    Illegal PstI site found at 1515
    Illegal PstI site found at 2022
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 95
    Illegal PstI site found at 493
    Illegal PstI site found at 1515
    Illegal PstI site found at 2022
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 725
    Illegal BsaI site found at 883
    Illegal BsaI site found at 2432
    Illegal BsaI.rc site found at 547


[edit]
Categories
Parameters
n/aBovine Pancreatic DNase I (in yeast mt codon table)