Coding
REX_Coding

Part:BBa_K809502

Designed by: Xiaopeng Xu   Group: iGEM12_Shenzhen   (2012-09-24)


GAL promoter + SP_REX + ADH1 terminator

The REX is linked with signal peptide of Zim17, GAL promoter and ADH1 terminator. When expressed in cytoplasm, it will be located into mitochondrial matrix.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1470
    Illegal AgeI site found at 150
    Illegal AgeI site found at 1119
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 835


[edit]
Categories
//chassis/eukaryote
Parameters
chassisYeast