Coding

Part:BBa_K809331

Designed by: Luohao Xu   Group: iGEM12_Shenzhen   (2012-09-26)


GAL promoter + YFP + SPtom40 + Toc75+ ADH1 terminator

BioBricks of Toc75_SPtom40 (K809327) is linked with GAL promoter and ADH1 terminator. When expressed in cytoplasm, The Toc75 will be directed on mitochondrial outer membrane as a channel protein, and the YFP can function as a reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1224
    Illegal PstI site found at 1763
    Illegal PstI site found at 2282
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3068
    Illegal PstI site found at 1224
    Illegal PstI site found at 1763
    Illegal PstI site found at 2282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2506
    Illegal BamHI site found at 2933
    Illegal XhoI site found at 1526
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1224
    Illegal PstI site found at 1763
    Illegal PstI site found at 2282
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1224
    Illegal PstI site found at 1763
    Illegal PstI site found at 2282
    Illegal NgoMIV site found at 2105
    Illegal NgoMIV site found at 3074
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2642
    Illegal BsaI.rc site found at 1974
    Illegal SapI site found at 687
    Illegal SapI.rc site found at 1485
    Illegal SapI.rc site found at 2714


[edit]
Categories
Parameters
n/aGAL promoter + YFP + SPtom40 + Toc75+ ADH1 terminator