Coding

Part:BBa_K809330

Designed by: Luohao Xu   Group: iGEM12_Shenzhen   (2012-09-26)


GAL promoter + YFP + SPtom70 + Toc34+ ADH1 terminator

BioBricks of Toc34_SPtom70 (K809324) is linked with GAL promoter and ADH1 terminator. When expressed in cytoplasm, The Toc34 will be directed on mitochondrial outer membrane, and the YFP can function as a reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1469
    Illegal XbaI site found at 809
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1469
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1469
    Illegal XhoI site found at 1657
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1469
    Illegal XbaI site found at 809
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1469
    Illegal XbaI site found at 809
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 558
    Illegal SapI.rc site found at 831


[edit]
Categories
Parameters
n/aGAL promoter + YFP + SPtom70 + Toc34+ ADH1 terminator