Coding
Toc75_SPto

Part:BBa_K809326

Designed by: Luohao Xu   Group: iGEM12_Shenzhen   (2012-09-26)


signal peptide of Tom40 + Toc75

Toc75 is linked with signal peptide of Tom40, thus can be directed on mitochondrial outer membrane as a beta-barrel protein

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 629
    Illegal PstI site found at 1168
    Illegal PstI site found at 1687
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2473
    Illegal PstI site found at 629
    Illegal PstI site found at 1168
    Illegal PstI site found at 1687
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1911
    Illegal BamHI site found at 2338
    Illegal XhoI site found at 931
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 629
    Illegal PstI site found at 1168
    Illegal PstI site found at 1687
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 629
    Illegal PstI site found at 1168
    Illegal PstI site found at 1687
    Illegal NgoMIV site found at 1510
    Illegal NgoMIV site found at 2479
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2047
    Illegal BsaI.rc site found at 1379
    Illegal SapI site found at 130
    Illegal SapI.rc site found at 890
    Illegal SapI.rc site found at 2119


[edit]
Categories
Parameters
None