Coding

Part:BBa_K809325

Designed by: Luohao Xu   Group: iGEM12_Shenzhen   (2012-09-26)


YFP + signal peptide of Tom70 + Toc34

Toc34 is linked with signal peptide, thus can be directed on mitochondrial outer membrane as a N-terminally anchored protein, and the YFP can function as a reporter

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 816
    Illegal XbaI site found at 156
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 816
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 816
    Illegal XhoI site found at 1004
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 816
    Illegal XbaI site found at 156
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 816
    Illegal XbaI site found at 156
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 178
    Illegal SapI.rc site found at 1512


[edit]
Categories
Parameters
None