Coding

Part:BBa_K809323

Designed by: Luohao Xu   Group: iGEM12_Shenzhen   (2012-09-26)


GAL promoter + YFP_SPtom22 + ADH1 terminator

The YFP is linked with signal peptide of tom22, GAL promoter and ADH1 terminator. When expressed in cytoplasm, it will be assembled on mitochondrial outer membrane as a tail-anchored protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None