Coding

Part:BBa_K809321

Designed by: Luohao Xu   Group: iGEM12_Shenzhen   (2012-09-26)


GAL promoter + YFP_SPtim21 + ADH1 terminator

The YFP is linked with signal peptide of Tim21, GAL promoter and ADH1 terminator. When expressed in cytoplasm, it will be located into mitochondrial inner membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aGAL promoter + YFP_SPtim21 + ADH1 terminator