Coding

Part:BBa_K809311

Designed by: Luohao Xu   Group: iGEM12_Shenzhen   (2012-09-23)


GAL promoter + RFP_SPtom70 + ADH1 terminator

The RFP is linked with signal peptide of Tom70, GAL promoter and ADH1 terminator. When expressed in cytoplasm, it will be assembled on mitochondrial outer membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
    Illegal AgeI site found at 1206
    Illegal AgeI site found at 1318
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 558


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Categories
Parameters
None