Part:BBa_K808004

tctA_505: large subunit A2 of the tripartite tricarboxylate transporter family

The large subunit A2 of the tripartite tricarboxylate transporter family (tctA_505, 52,99 kDa) was isolated from Comamonas testosteroni KF-1. The tripartite tricarboxylate transporter system consists of three different proteins: a periplasmatic solute binding receptor, a membrane protein with 12 putative transmembrane alpha-helical spanners (in this case tctA_503), and a small poorly conserved membrane proteine with four putative transmembrane alpha-helical spanners^[1].The strain was purchased from Leibniz Institute DMSZ-German Collection of Microorganism and Cell Cultures (DMSZ no. 14576). The original sequence contains a Pst1 recognition site. To eliminate this recognition site a directed-site mutagenic PCR was performed. (For more details [http://2012.igem.org/Team:TU_Darmstadt/Protocols/mutagenic_PCR mutagenic PCR]). To characterized the structure of the tctA_505 bioinformatic tools like Protein Homology/anologY Recognition Engine V 2.0 (PHYRE2), I-TASSER servers, and TMHMM was used. The TMHMM predicted a transmembrane protein with 11 alpha-helical spanners (Fig. 1).The NCBI Protein BLAST results shows that the tctA_505 subunit A2 belongs to the tctA superfamily.Phyre2 an I-Tasser server homology modelling did not give a significant result for the structure of the tctA_505 large subunit A2. ]]

Figure 1. TMHMM prediction of the tctA_505 subunit A2. It shows 10 alpha-helical spanners.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 557
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 193
  Illegal NgoMIV site found at 802
  Illegal NgoMIV site found at 1186
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Sasoh, M., E. Masai, et al. (2006). "Characterization of the terephthalate degradation genes of Comamonas sp. strain E6." Appl Environ Microbiol 72(3): 1825-1832.

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• Winnen, B., R. N. Hvorup, et al. (2003). "The tripartite tricarboxylate transporter (TTT) family." Res Microbiol 154(7): 457-465.
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