Deleted
Sample Discontinued
Experience: None
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Coding
LEV

Part:BBa_K807002

Designed by: Ian Roney   Group: iGEM12_uOttawa_CA   (2012-09-28)


LEV: LexA DNA Binding Domain - Human Estrogen Receptor - VP16 Activating Domain

This part is a synthetic transcriptional activator with specificity for the LexA operator site. It is inactive in its native form but can be activated by the addition of Beta-Estradiol. The Beta-Estradiol acts to dimerize the activator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 250
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 112
    Illegal BglII site found at 675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 575
    Illegal BsaI.rc site found at 47


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