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Coding
GEV

Part:BBa_K807000

Designed by: Ian Roney   Group: iGEM12_uOttawa_CA   (2012-09-28)


GEV - Gal4DNA Binding Domain-Human Estrogen Receptor - VP16 Activation Domain

This part is a synthetic transcriptional activator with specificity for the Gal4 upstream activating sequence that can be found in the Gal1 promoter of Saccharomyces cerevisiae. It is inactive in its native form but can be activated by the addition of Beta-Estradiol. The Beta-Estradiol acts to dimerize the activator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 701
    Illegal XhoI site found at 218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137
    Illegal BsaI site found at 601


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