Coding

Part:BBa_K806002

Designed by: Chang Ye   Group: iGEM12_SYSU-China   (2012-09-24)


hda - regulator of DnaA that prevents premature reinitiation of DNA replication

We find a better way to slow down the cell cycle of E.coli,by stop the initiation of DNA replication. 

  Hda is a great candidate for stop replication initiation.[1]

  we cloned and overexpressed it to slow down certain kind of cells in a mix quorum system, in order to maintain a more stable system and achieve better energy partition.

[1] Elliott Crooke et al.EMBO report. Hda inactivation of DnaA is the predominant mechanism preventing hyperinitiation of Escherichia coli DNA replication.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 330
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 330
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 330
    Illegal BglII site found at 225
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 330
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None