Coding

Part:BBa_K801039

Designed by: Dong-Jiunn Jeffery TRUONG   Group: iGEM12_TU_Munich   (2012-09-21)

SV40NLS-GAL4AD-linker-PIF3 (part for GAL4/LexA based light-switchable promoter system)

CDS for a synthetic hybrid fusion protein. This part is the second component of a GAL4/LexA based yeast light-switchable-promoter system (BBa_K801042, BBa_K801043, BBa_K801040, BBa_K801041). This part is flanked by RFC10 pre and suffix.

Background

Principle of light-dependent switching of gene-expression.

























Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 407
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
//proteindomain
//proteindomain/activation
//proteindomain/binding
//proteindomain/localization
Parameters
None