Part:BBa_K801038

PhyB-linker-Gal4bd

Composite part of BBa_K801031 and BBa_K801037. This fusion protein codes for a part (of two parts) of an GAL4 based light-switchable promoter system. This GAL4DBD of this part is responsible for binding of GAL4 responsive elements like the upstream activating sequence (UAS) of pGAL1. The PhyB part is responsible for the direct recruitment of PIF3 (phytochrome interacting factor 3) and hence responsible for the indirect recruitment of the transcriptional activation domain GAL4AD because PIF3 is fused to GAL4AD (BBa_K801039).

It is known that there is already a endogenous nuclear localization signal in the N-terminus of GAL4DBD but to ensure nuclear translocation of the fusion protein there is an improved part of this which has an additional SV40 nuclear localization signal in the N-terminus of this part (BBa_K801040).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 487
  Illegal BamHI site found at 569
  Illegal XhoI site found at 520
  Illegal XhoI site found at 539
  Illegal XhoI site found at 2674
  Illegal XhoI site found at 3008
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2059
  Illegal BsaI site found at 2465
  Illegal BsaI site found at 2927
  Illegal SapI site found at 736