Phytochrome B (PhyB, first 908 N-terminal amino acids)
Improved version of BBa_K365002 with RFC25 pre- and suffix to to permit N- and C-terminal protein fusions. For example an SV40 nuclear localization signal (BBa_K801030) can be fused to the N-terminus of this part.
Phytochrome naturally incorporates phycocyanobilin (PCB) to enable its photoconvertible ability to bind and unbind PIF3 (phytochrome interacting factor 3, BBa_K365000 (essential first 100 amino acids of PIF3 to allow binding to PhyB).
When exposed under red light (650–670 nm), PhyB changes its conformation to the active state Pfr. The Pfr of PhyB is now able to bind PIF3. The t1/3 of the active state Pfr is about ~30 min, after this time half of the quantity is reversed into its ground state Pr. Photoreconversion to its ground state is also immediately possible by far-red light exposition (705-740 nm).
With this light-switchable ability, one can create light-switchable protein recruitment, e. g. for light-switchable promoter systems (BBa_K801042 and/or BBa_K801043)
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 487
Illegal BamHI site found at 569
Illegal XhoI site found at 520
Illegal XhoI site found at 539
Illegal XhoI site found at 2674
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 2059
Illegal BsaI site found at 2465
Illegal SapI site found at 736