Part:BBa_K801031

Phytochrome B (PhyB, first 908 N-terminal amino acids)

Improved version of BBa_K365002 with RFC25 pre- and suffix to to permit N- and C-terminal protein fusions. For example an SV40 nuclear localization signal (BBa_K801030) can be fused to the N-terminus of this part.

Phytochrome naturally incorporates phycocyanobilin (PCB) to enable its photoconvertible ability to bind and unbind PIF3 (phytochrome interacting factor 3, BBa_K365000 (essential first 100 amino acids of PIF3 to allow binding to PhyB).

When exposed under red light (650–670 nm), PhyB changes its conformation to the active state P_fr. The P_fr of PhyB is now able to bind PIF3. The t_1/3 of the active state P_fr is about ~30 min, after this time half of the quantity is reversed into its ground state P_r. Photoreconversion to its ground state is also immediately possible by far-red light exposition (705-740 nm).

With this light-switchable ability, one can create light-switchable protein recruitment, e. g. for light-switchable promoter systems (BBa_K801042 and/or BBa_K801043)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 487
  Illegal BamHI site found at 569
  Illegal XhoI site found at 520
  Illegal XhoI site found at 539
  Illegal XhoI site found at 2674
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2059
  Illegal BsaI site found at 2465
  Illegal SapI site found at 736