Terminator

Part:BBa_K799029

Designed by: Daniel Wolozny   Group: iGEM12_Johns_Hopkins-Wetware   (2012-09-25)

MFA2 Terminator

Terminator of the MFA2 gene from Saccharomyces cerevisiae. It confers relative instability to the mRNA produced, thus reducing the amount of mRNA retained in the cell and possibly reducing the production of protein. https://www.jstage.jst.go.jp/article/bbb/75/11/75_110246/_pdf "TPS1 Terminator Increases mRNA and Protein Yield in a Saccharomyces Cerevisiae Expression System"

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 217

Plasmid Map

MFA2t plasmid.png

Sequencing

MFA2t sequence.png

This is the sequencing result for the MFA2 gene terminator. As can be seen, it is a perfect match to the expected sequence.

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Categories
Parameters
None