Coding

Part:BBa_K799028

Designed by: Daniel Wolozny   Group: iGEM12_Johns_Hopkins-Wetware   (2012-09-25)

typeIIS-less GFP

GFP coding sequence in which naturally occurring BsaI sites have been re-coded. The encoded protein sequence remains unchanged and works as expected.

This GFP coding sequence (CDS) is compatible with BsaI-dependent assembly strategies, such as that described in RFC88 - Yeast Golden Gate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Plasmid Map

GFP bsa- plasmid.png

Sequencing

GFP no bsa sequencing.png

This is the sequencing result for BsaI-less GFP coding sequence. As can be seen, it is a perfect match to our expected sequence.

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Categories
Parameters
None