Regulatory

Part:BBa_K799001

Designed by: Jiarui Wang   Group: iGEM12_Johns_Hopkins-Wetware   (2012-09-23)

Ethanol/Stress Inducible YJL200C Promoter

YJL200C promoter is expected to drive gene expression in Saccharomyces cerevisiae in the presence of ethanol.

We tested this by expressing GFP under the control of YJL200C promoter in the presence of multiple different concentrations of ethanol. We found an induction of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 172
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 172
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 172
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

ProA1 fluoro.png

This is fluorescence data for the expression of GFP under the control of YJL200C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.

Plasmid Map

YJL200C plasmid.png

Sequencing

YJL200C sequencing.png

This is the sequencing result for YJL200C promoter. As can be seen, it is a perfect match to the genomic DNA promoter.

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