DNA

Part:BBa_K798011

Designed by: Taijie Jin   Group: iGEM12_Fudan_D   (2012-09-24)

Ribozyme sequence

This part is a ribozyme sequence whose design is based on the phenomena that structured RNAs embedded in the untranslated regions (UTRs) of messenger RNAs can regulategene expression(Martick, Horan et al. 2008). Itcan be insertedwith its target substrate sequence into the 5’UTR region of any genes and as a result down-regulate theexpression of the gene or even entirely silence the gene. The ribozyme sequence should be after the substrate sequence. Besides, the length of interval sequence between ribozyme and substrate is alterable from 0 to nearly 1000 bp.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None